Abstract: ObjectiveTo investigate the expression of AnnexinⅡand S100A4 in papillary thyroid carcinoma and their correlations with the occurrence and lymphatic metastasis of papillary thyroid carcinoma. Methods The immunohistochemistry S-P and in situ hybridization technique was used to test the different expressions of AnnexinⅡ,S100A4 proteins and AnnexinⅡ mRNA in tissues of 50 papillary thyroid carcinoma,14 thyroid adenoma,26nodular goiter and 10 normal thyroid gland. Results The positive expression rates of AnnexinⅡ,S100A4 proteins and AnnexinⅡmRNA in papillary thyroid carcinoma were 82.00%(41/50),78.00%(39/50) and 74.00%(37/50), respectively.They were significantly higher than those in thyroid adenoma，nodular goiter and normal thyroid gland(P＜0. 05). The expression rates of AnnexinⅡ,S100A4 proteins and AnnexinⅡmRNA in papillary thyroid carcinoma were associated with lymph node metastasis(P＜0. 05). The expression of AnnexinⅡprotein was positive correlated with that of S100A4 protein(r=0.380,P=0.025).The expression of AnnexinⅡprotein was positive correlated with that of AnnexinⅡmRNA (r=0.434,P=0.008). Conclusion The over expressions of AnnexinⅡand S100A4 were closely related with the occurrence and lymphatic metastases of thyroid carcinoma.Combining detection of AnnexinⅡ and S100A4 would help to distinguish the benign or malignant disease of thyroid gland and predict tumor development and prognosis.