雷帕霉素上调Beclin 1诱导肝癌细胞死亡的体外研究

Rapamycin Induces Cell Death of Hepatoma Cells via Upregulation of Beclin 1

摘要: 目的研究雷帕霉素诱导肝癌细胞死亡的方式以及时效与量效关系。方法锥虫蓝排斥实验分析雷帕霉素诱导肝癌细胞死亡的时效、量效关系；透射电镜技术检测雷帕霉素诱导肝癌细胞死亡方式；Western blot检测Beclin 1表达变化。结果雷帕霉素诱导肝癌细胞死亡具有时效和量效关系；可诱导肝癌细胞的凋亡和自噬性细胞死亡；可上调Beclin 1蛋白的表达。结论雷帕霉素可能是一种新的有效的肝癌化疗药物。

Abstract: ObjectiveTo investigate the rapamycin-induced cell death and the time and dose-effect relationship. MethodsCell death ratio was analyzed by using trypan blue exclusion assay. The rapamycin-induced cell death fashion was detected by TEM in SMMC7721. Furthermore,Beclin 1 was detected by using Western blot. ResultsRapamycin significantly induces liver cell death,and has significant time and dose-effect dependence. Rapamycin can induce both apoptosis and autophagy in SMMC7721. Beclin 1 was upregulated by rapamycin. ConclusionRapamycin can induce apoptosis and autophagic cell death in hepatoma cells,Beclin 1 upregulation may be the direct cause for rapamycin -induced cell death in HCC cells.