Abstract: ObjectiveTo observe the effect of Xiaoaiping on expression of adhesion molecule-1 in mice. MethodsLiver cancer cells(H22) were transplanted subcutaneously into Kunming mice right armpit to establish experimental model. 50 mice were randomly divided into 5 groups as well as normal group .XAP with 0.01 ml/（g·d), 0.02 ml/（g·d) and 0.04 ml/（g·d) doses (low, medium and high doses) was intraperitoneally injected d1~14. The negative control treated by saline, and positive control group was injected 5-Fu by 5~20 mg/(kg·d) d1~7. ELISA was used to test sICAM- 1 level, calculation spleen index and inhibition rate. Results The tumor inhibition rates in low, medium and high-dose groups injected with XAP were 20.7%, 31.6%, 38.63% respectively, and higher than that in negative control group, while the high-dose group was similar to positive control group (45.97%). Compared with negative control group, spleen index in each research groups were increased statistically (P<0.05); and similar to positive control group (P>0.05). Secretion capacities in Low, medium and high-dose group were (53.26±9.36) ng/L,(35.75±11.16) ng/L,(29.13±8.52)ng/L respectively and more decreased, compared with negative group (P<0.05). No obvious difference was observed between the high-dosage group and the positive group (P>0.05). Conclusion Xiaoaiping inhibited tumor growth, and improve immune function, through reducing the expression of serum cell adhesion molecule-1 in tumor -bearing mice.