高良姜素诱导肺癌A549细胞凋亡的研究

doi:10.3971/j.issn.1000-8578.2011.11.003

高良姜素诱导肺癌A549细胞凋亡的研究

Galangin Induces Apoptosis on Lung Cancer A549 Cells

摘要

摘要: 目的研究高良姜素诱导肺癌细胞系A549细胞凋亡效应。方法MTT法测定A549细胞生长抑制率，荧光显微镜观察细胞形态学的变化，流式细胞仪分析细胞周期和线粒体膜电位的变化。Western blot检测细胞凋亡相关蛋白的表达变化。结果随高良姜素浓度增高，A549细胞生长抑制率明显上升，IC50在30.15 mg/L。细胞凋亡可在10~80 mg/L 高良姜素处理后24 h出现，呈浓度依赖性。高良姜素使线粒体膜电位降低。Caspases被激活，Bcl-2、Bcl-XL 表达下调，p53、Bax、Bid表达呈浓度依赖性上调。结论高良姜素可能是通过线粒体途径诱导A549细胞发生凋亡。

Abstract: Objective To investigate apoptosis induced by Galangin in lung cancer A549 cells. Methods Cell inhibition ratio was assayed by MTT method. Morphologic assessment of apoptosis was performed with a fluorescence microscope,and cell cycle and mitochondrial membrane potential were analyzed by flow cytometry.The expression levels of proteins were measured by Western blot. ResultsA549 cells were inhibited by Galangin in a dose-dependent manner.IC50 of Galangin was 30.15 mg/L. The apoptosis appeared in 24 hours after treated by Galangin. Morphologic changes including nuclear chromatin condensation fluorescence strength was observed through fluorescence microscope.Caspases were activated.The expression of Bcl-2 and Bcl-XL decreased，while the expression of p53,Bax and Bid increased. ConclusionGalangin could induce A549 cell apoptosis through mitochondria pathway.

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