Abstract: Objective To investigate epidermal growth factor receptor(EGFR),tumor suppressor of non -small cell lung cancer 1(TSLC1)in the throat benign lesions and precancerous lesions and cancer of the larynx in both the rule and expression analysis of the organization. Explore glottis of mechanisms that provide for laryngeal cancer prevention. Methods Throat benign lesions,and laryngeal precancerosis with 30 cases,using immunohistochemical staining (SP) detection in which the expression of EGFR,TSLC1. Results From throat benign lesions → precancerous lesions → laryngeal of the larynx,the increasing trend of EGFR expression,the expression TSLC1 dropped,the difference is statistically (P<0.01).Laryngeal EGFR,TSLC1 expression without correlation(P>0.05). Conclusion EGFR and TSLC1 in the throat by a benign lesion to precancerous lesions and cancer of the development process may play an important role.