Abstract: ObjectiveTo observe apoptosis inhibition of survivin gene on laryngeal carcinoma cells Hep2 as well as to study protein profile changes by SELDI-TOF-MS（surface enhanced laser desorption/ionization time-of-flight mass spectrometry）in vitro. Methods Recombinant adenovirus vector of survivin was collected to infect laryngeal carcinoma cells Hep2. Its inhibiting effect on apoptosis was detected by MTT，FACS and Western. Infected cells were collected and cellular protein profile was analyzed by SELDI-TOF-MS. Results Two days after transfection, survivin adenovirus infected cells grew faster than those infected with blank adenovirus.FACS analysis showed that G1/S and G2/M phase cells remarkably increased with percentage of 26% of G2/M in survivin adenovirus infection. The expressions of apoptosis related proteins CASPASE-3, CASPASE-10 and CASPASE-11 decreased in the infected tumor cells. SELDI technique was used to detect cellular protein profile changes after infection. The peaks of M4924_02，M8518_09 and M2454_31 protein in SELDI decreased remarkably in the infection group. Conclusion survivin adenovirus could inhibit apoptosis in the human laryngeal carcinoma cell line Hep2, and there was a correlation with proteins Caspase-3, Caspase-10 and Caspase-11.