Abstract: ObjectiveTo re-evaluate the diagnostic value of multi-tumor marker protein biochip detective system for liver cancer，and to guide the proper application of the detection method in clinical diagnosis. MethodsUsing the multi-tumor markers protein biochip detective system to determine and analyze the concentration values of 12 tumor markers （CA19-9， NSE， CEA， CA242， CA125，CA153， AFP， Ferritin， f-PSA， PSA， β-HCG， HGH） in the serum levels of 99 liver cancer patients，93 benign liver disease patients and 90 healthy persons. ResultsBy statistical analysis, we found that the concentrations of AFP， Fer，CA19-9，CA242 were all higher in the PHC group than in the non-PHC groups；the difference was significant；combined measurement of the above-mentioned four kinds of indicators, the positive ratio was 75%, had higher sensitivity than single tumor marker in the detective system. ConclusionIn this study, adopting the multi-tumor marker protein chip detection system, the sensitivity of combined detection of liver cancer was only increased to 75%, lower than the sensitivity of 82.89%~91.6%,compared to more articles.