Wnt1、β-catenin、APC和cyclin D1蛋白在胃癌中的表达

doi:10.3971/j.issn.1000-8578.2011.02.012

Wnt1、β-catenin、APC和cyclin D1蛋白在胃癌中的表达

Expression of Wnt1，β-catenin，PC and cyclinD1 Proteins in Gastric Carcinoma

摘要

摘要: 目的检测Wnt1、β-catenin、APC和cyclin D1蛋白在胃癌中的表达情况。方法对45例人胃癌组织标本及肿瘤边缘5cm以外的正常组织进行LSAB免疫组织化学检测。结果45例胃癌组织中Wnt1、β-catenin、cyclin D1阳性率分别为57.78％、73.33％和51.11％，正常对照组织几乎不表达(β-catenin正常组织膜表达)，相比差异有统计学意义（P<0.01）；APC 在GC（gastric carcinoma,GC）中表达的阳性率为（53.33%），低于正常组织（91.11％），差异具有统计学意义（P<0.01）。在同一胃癌标本中，Wnt1、β-catenin、cyclin D1同时高表达，即Wnt通路处于活化状态(Wnt+)占44.44％(20/45)，正常组织未见激活状态（0/45），差异具有统计学意义（P<0.01）。结论Wnt1-β-catenin-APC-cyclin D1通路在胃癌发生过程中存在异常表达。

Abstract: ObjectiveTo detect the expression of Wnt1，β-catenin，APC and cyclin D1 proteins in human gastric carcinoma. Methods The immunohistochemical staining(LASB) was performed in 45 specimens and normal tissue adjacent to tumor border (>5cm). Results In 45 cases gastric carcinoma，the positive rates of Wnt1，β-catenin and cyclin D1 were 57.78％,73.33％ and 51.11％ respectively. In comparison，statistic difference is apparent because of their absence (excluding membrane expression of β-catenin) in normal tissue (P<0.01).However，APC expression is lower in gastric carcinoma than that in normal tissue (53.33% vs. 91.11％). There is marked significance likewise（P<0.01）.The co-expression (Wnt pathway is activated，Wnt+) rate of Wnt1，β-catenin and cyclin D1 is 44.44％，which is much higher than that in normal tissue（P<0.01）. Conclusion Wnt1-β-catenin-APC-cyclinD1 signal pathway is abnormal expressed in gastric carcinoma genesis.

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