Abstract: Abstract:Objective To investigate the molecular mechanism at proteomic level of Fei-Yan-Ning Granule in nude mice with transplanted human lung carcinoma. Methods Nude mice with transplanted human lung carcinoma cell line H460 were randomly divided into control group and Feiyanning Granule treated group (FYN group). The total protein was isolated from carcinoma tissue of groups,and the changes of proteins expression profile of tumor tissues were evaluated by two-dimensional electrophoresis matrix-assisted laser desorption ionization-time of flight mass spectrometry and tandem mass spectrometry. Results As compared with the control group, eighteen differentially expressed proteins were identified unambiguously, among which 9 proteins were significantly up-regulated and 9 proteins were down-regulated, involving multiple scopes such as cell cycle, signal transduction, energy metabolism, protein synthesis,and cell receptors. Conclusion The antitumor effect of Feiyanning granule is multi-targeted, which elucidates part of the pharmacologic mechanism of Feiyanning granule at proteomic level.