Abstract: Abstract: Objective To study the effect of rotary magnetic field (RMF) combining 5-Fu on the cycle and apoptosis of mouse cell line SP2/0 in vitro. Methods SP2/0 cells were randomly divided into four groups: control group (N), 5-Fu group (C), magnetic group (M) and magnetic combining 5-Fu group (M+C).The M and M+C groups were treated with a RMF for two hours once a day.On day 4, the C and M+C groups were treated with 5-Fu 20 μg/ml.On day 5, cell cycle and apoptosis were measured by the flow cytometric (FCM). Results The S phase proportion of the M group and the G1 phase proportion of the C group were higher than that of the other three groups（P<0.05）.The S phase proportion of the M+C group decreased and lower than that of the M group，but was still higher than that of the N and C groups（P<0.05）.There was no significant difference in apoptosis rates between the N and M groups（P>0.05）.The apoptosis rates of the C and M+C groups were remarkedly higher than those of the N and M groups and the M+C group had the highest apoptosis rate. Conclusion The RMF can't induce the apoptosis.But it can enhance the cytotoxicity of 5-Fu and promote the cell apoptosis.The mechanism of the apoptosis may be related to SP2/0 cell line arrested at S phase.Objective Cervical carcinoma clinical stages associated proteins would be found by comparing differential expressed proteins from differential clinical stage cervical carcinoma tissues. Methods Total protein from cervical carcinoma tissues was extracted; differential proteome profiles were established and analysized by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis(2D-PAGE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS).HSP70 and Galectin-7 expression were assayed by Western Blot and immunohistochemical technique. Results Well-resolved reproducible 2-DE profiles of human cervical carcinoma tissues were obtained.Average protein dots were(1 098±23),(1 142±21) at early-stage and medium-advanced stage respectively; By analysis with mass spectrometry and bioinformatics, 12 of them were well characterized.4 proteins were over-expressed in early-stage group, including Hemoglobin subunit beta HB,caspase-14,Galectin-7,CK19; 8 protein were over-expressed in medium-advanced stage, including Lamin-B1,Flavin reductase、Glutamate dehydrogenase 1,Retinal dehydrogenase 1,NMP238,HSP70,Calmodulin-like 5,S100A9.HSP70 expressed higher at medium-advanced stage group than at early-stage group and Galectin-7 expressed higher at early-stage group than at medium-advanced group. Conclusion Differential expressed proteins exist in clinical stage of cervical carcinoma, which would be biomarkers for diagnosis and prediction of prognosis.