20（S）-人参皂苷Rg3对血管内皮细胞增殖和迁移的抑制作用
Inhibitory Effect of 20(s)-Ginsenoside Rg3 on Proliferation and Migration of Vascular Endothelial Cells
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摘要: 目的 探讨20（S）-人参皂苷Rg3（SPG-Rg3）抗肿瘤诱导新生血管生成的作用机制。方法 采用MTT法、PCNA免疫荧光染色和Boyden小室迁移实验，观察SPG-Rg3对B16黑色素瘤细胞条件培养液（Conditioned Medium of B16 Melanoma Cells, BMCM）诱导的人脐静脉内皮细胞增殖和迁移的影响；并通过免疫细胞化学染色法观察了SPG-Rg3对B16黑色素瘤细胞血管内皮细胞生长因子（Vascular Endothelial Growth Factor, VEGF）及金属基质蛋白酶-9（Matrix Metalloproteinase-9, MMP-9）表达的影响。结果SPG-Rg3组BMCM的促内皮细胞增殖和迁移作用均明显弱于对照组，且SPG-Rg3浓度为5μg/ml时能够降低B16黑色素瘤细胞VEGF及MMP-9的表达。结论SPG-Rg3通过减少肿瘤细胞分泌细胞因子VEGF和MMP-9，抑制肿瘤细胞对血管内皮细胞增殖和迁移的促进作用，从而间接发挥其抗肿瘤新生血管生成的作用。Abstract: Abstract:Objective To study the mechanism of inhibitory effect of ginsenoside SPG-Rg3 on angiogenesis. Methods To observe the effect of SPG-Rg3 on the proliferation and migration of human umbilical vein endothelial cells induced by conditioned medium of B16 melanoma cells (BMCM) by MTT assay, PCNA immunofluorescence staining and migration assay.The VEGF and MMP-9 expression of B16 melanoma cells treated with SPG-Rg3 were analyzed by immunocytochemical staining. Results The promoting effect of BMCM on proliferation and migration of vascular endothelial cells was reduced in SPG-Rg3 groups.Moreover, 5μg/ml SPG-Rg3 inhibited the VEGF and MMP-9 expression of B16 melanoma cells. Conclusion SPG-Rg3 can suppress the promoting effect of B16 melanoma cells on proliferation and migration of vascular endothelial cells by decreasing secretion of VEGF and MMP-9, thus indirectly inhibited tumor angiogenesis.
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