Abstract: Objective：To investigate the application of the uterine manipulator in the female Laparoscopic rectectomy. Methods：Using the uterine manipulator to raise the womb to expose the Douglas fossa in order to provide a good vision in 38 cases of female Laparoscopic rectectomy. Results：The 38 cases treated by such operation were successful, including Dixon 34 cases(2 cases combined with hysteromyomectomy, 2 cases of removal of ovarian cysts, 1 case of partial resection of the bladder) and 4 cases of Miles, 1 case of vaginal posterior wall injury, 1 case of postoperative anastomotic bleeding， no other peri-operative complications. The average operation time was (170±40)min, the estimated operative blood loss was (35±10)ml, the number of removed matastatic lymph nodes were (14±4)， postoperative hospitalization term was (9±1) days, without conversion. Conclusion：The approach using the uterine manipulator to replace the Uterine suspension can provide a better vision in female Laparoscopic rectectomy， and it is convenient, flexible and less traumatic.