基质金属蛋白酶-24在卵巢浆液性囊腺癌细胞株中的表达

Expression of Matrix Metalloproteinase-24 in Ovarian Serous Cystadenocarcinoma Cell Lines

摘要: 目的探讨基质金属蛋白酶－24(MMP-24)基因在卵巢癌细胞株中的表达及临床意义。方法应用半定量RT-PCR和Western blot对卵巢浆液性囊腺癌细胞株OVCa-R₃、SKOV₃ 、HO-8910 及人卵巢细胞株TC-1进行检测。结果 3种卵巢癌细胞株中MMP-24基因和蛋白的表达均增强，与正常卵巢细胞相比，差异有统计学意义。结论 MMP-24基因可能与卵巢癌的发生、发展有关。

Abstract: Objective To investigate the expression of matrix metralloproteinase-24 (MMP-24) gene and MMP-24 protein in ovarian serous cystadenocarcinoma cell lines. Methods The expression of MMP-24 gene and MMP-24 protein of human ovarian serous cystadenocarcinoma cell lines HO-8910、OVCa-R₃、SKOV₃ were analyzed with RT-PCR and Western blot respectivly. Results High levels of MMP-24 gene and MMP-24 protein were observed in ovarian carcinoma cell lines HO-8910、OVCa-R₃、SKOV₃,which were significantly higher than those in normal ovarian cells. Conclusion The MMP-24 gene possibly have an important association with the occurrence and development in ovarian carcinoma.