新城疫病毒D817株体外高效杀伤肝癌细胞及其作用机制

doi:10.3971/j.issn.1000-8578.2010.03.017

新城疫病毒D817株体外高效杀伤肝癌细胞及其作用机制

Cytotoxic Activities ofNOV D817on Hepatocellular Carcinoma Cells in Vitro and Their Possible Mechanism

摘要

摘要: 目的与NDV疫苗株LaSota对比研究一株NDV D817株对肝癌细胞高效特异性的杀伤效应和作用机制，进一步筛选NDV溶瘤毒株。方法用MTT法对比病毒对三株传代肝癌细胞株SMMC-7721、Bel-7404和HepG-2及一株正常肝细胞株HL-7702的杀伤效应，并用TUNEL法及透射电子显微镜观察病毒诱导肿瘤细胞发生凋亡作用。结果 NDV D817株对肝癌细胞株SMMC-7721、Bel-7404和HepG-2杀伤效应高达80％，显著高于疫苗LaSota株（P<0.01），而对人正常肝细胞HL-7702无明显影响；病毒在肝癌细胞中明显复制增殖，对细胞的杀伤活性与病毒作用剂量和病毒作用时间成正比；NDV D817株有效诱导肝癌细胞发生凋亡。结论 NDV D817株有效诱导肝癌细胞发生凋亡，对SMMC-7721、Bel-7404和HepG-2细胞具有高效杀伤性，而对正常肝细胞HL-7702未见明显影响，推测为溶瘤株。

Abstract: Objective To investigate the cytotoxiciactivities of Newcastle disease virus(NDV) D817 and lasota to hepatocarcinoma cell. Methods The cytotoxic effects of two NDV strains on the SMMC-7721，Bel-7404， HepG-2 and HL-7702 Cell lines were investigated using MTT assay. The apoptosisofhepatocarcinoma cellinducedbyNDV strain D817was evaluatedby TdT-mediated dUTP nick end labeling(TUNEL) and transmission electron microscopy. Results The apparently pathological changes were observed in SMMC-7721，Bel-7404 and HepG-2 tumor cells, but not in HL-7721 cells. TheNVD-strain D817 exhibitedhigher suppressed effect on tumor cellsthan the LaSota. Conclusion NDV-strain D817 couldeffectivelyinduce theapoptosisofhepatocarcinoma cell, which will be a potential anticancer agent for the treatment of liver cancer.

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