组蛋白乙酰化在二烯丙基二硫抑制CNE2细胞生长增殖中的作用

doi:10.3971/j.issn.1000-8578.2010.03.003

组蛋白乙酰化在二烯丙基二硫抑制CNE2细胞生长增殖中的作用

Effect of Histone Acetylation on Proliferation of CNE2 Cells Inhibited by Diallyl Disulfide

摘要

摘要: 目的探讨二烯丙基二硫(DADS)在抑制CNE2细胞生长增殖中，组蛋白乙酰化状态的改变情况。方法采用MTT、细胞计数、细胞形态学和Western blot方法，分析DADS对CNE2细胞的增殖抑制作用及与其调控细胞组蛋白乙酰化水平的关系。结果 MTT法、细胞计数显示，DADS能明显抑制CNE2细胞增殖，细胞群体倍增时间延长，呈浓度和时间依赖性；HE染色显示，DADS处理CNE2细胞48h后，细胞异型性明显降低，核浆比明显减少；Western blot 分析表明，90μmol/L、140μmol/L、240μmol/L和400μmol/L DADS处理CNE2细胞48h后，组蛋白H3乙酰化的表达较对照组分别增加20%、32%、42%和30%，差异有统计学意义（P<0.05）；组蛋白H4乙酰化程度较对照组差异无统计学意义。结论 DADS可抑制CNE2细胞生长增殖，其作用机制可能与组蛋白H3乙酰化水平提高有关。

Abstract: Objective To explore the change conditions of histone acetylation on proliferation of CNE2 cells inhibited by diallyl disulfide (DADS). Methods The growth inhibitions of CNE2 cell line were measured by MTT assay and cell counting.Expression of H3 and H4 was determined by western blot method. ResultsThe results from MTT assay and cell counting showed that DADS significantly inhibited the growth of CNE2 cells.Light microscope examination indicated that the heteromorphism and karyoplasmic ratio decreased.The results from Western blot showed that the acetylation level of histone H3 in CNE2 cell line increased by 20%,32%,42% and 30% compared to the control group,respectively,when treated with 90,140,240 and 400 μmol/L DADS for 48 h.The express of H3 exhibited a significant difference in the DADS does dependence.However,the acetylation level of histone H4 did not changed with a change of DADS does. Conclusion The diallyl disulfide (DADS) showed antiproliferation to the cultured human nasopharyngeal carcinoma CNE2 cell line,which is related to the over-expression of histone H3 resulted from DADS.

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