Abstract: Objective To evaluate the relationship between the ^99mTc-MIBI Scintigraphy of ovarian cancer and the efficacy of chemotherapy. Methods Twenty-eight patients with advanced ovarian cancer underwent ^99mTc-MIBI cintigraphy. Static planar images were taken at 10 min and 60 min after scintigraphy． Meanwhile technique of region-of-interest (ROI) was used to calculate T/ N and retention indices of ^99mTc -MIBI．Each patient received chemotherapy of TP. Results The patients with low T/N and low retention indices had poor efficacy of chemotherapy. The patients with high T/N and high retention have good efficacy of chemotherapy. There was significant difference between two groups. Conclusion The ^99mTc-MIBI Scintigraphy may be used to evaluate the efficacy of chemotherapy in ovarian cancer，and thus can offer theoretical basis for individual therapy．