曲古菌素A对甲状腺癌细胞增殖及凋亡的影响

doi:10.3971/j.issn.1000-8578.2009.12.003

曲古菌素A对甲状腺癌细胞增殖及凋亡的影响

Effects of Trichostatin A on Inhibiting Growth and Inducing Apoptosis of Thyroid Cancer Cells in Vitro

摘要

摘要: 目的探讨组蛋白去乙酰化酶抑制剂曲古菌素A体外抑制甲状腺癌细胞增殖、诱导凋亡的作用。方法采用磺酰罗丹明B染色分析法、Hoechst33342/PI双染荧光显微镜检测及PI单染流式细胞仪分析等技术检测曲古菌素A对不同甲状腺癌细胞增殖、凋亡及细胞周期的影响。结果不同浓度的曲古菌素A处理48h，能明显抑制甲状腺癌细胞的增殖，并且随着药物浓度的增加抑制率也增大，各组间及与对照组相比差异均具有统计学意义（F=35.67, P＜0.01）；双染后镜下观察显示曲古菌素A使甲状腺癌细胞呈明显的凋亡状态，流式细胞仪分析则表明诱导后滤泡状甲状腺癌细胞凋亡峰(Sub-G1)比值升高，与对照组比较其差异有统计学意义（t=6.225, P＜0.01）。结论曲古菌素A能显著抑制甲状腺癌细胞体外的增殖及诱导甲状腺癌细胞的凋亡，其作用呈浓度和时间依赖性。

Abstract: Objective To investigate the effects of Trichostatin A on inhibiting growth and induing apoptosis of thyroid cancer cell lines in vitro. Methods The cytotoxic effects of Trichostatin A on thyroid cancer cells were determined by using SRB assay. DNA content was measured by PI staining through flow cytometry. Apoptosis of thyroid cancer cells induced by Trichostatin A was identified by fluorescence microscopy with Hoechst33342/PI staining. Results After incubation of thyroid cancer cells with Trichostatin A for 48h, the inhibiting rate, with the augment of drug concentration, increased significantly（F=35.67, P＜0.01）. Trichostatin A induced apoptosis of thyroid cancer cells with Sub-G1 phase peak increasing in flow cytometry（t=6.225, P＜0.01）. Conclusion Trichostatin A possesses inhibiting proliferation and apoptosis inducing activity on thyroid cancer cells. These effects appear to be dose-dependent.

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