Abstract: Objective To provide data for the early diagnosis and early treatment of cervical cancer by tracking and follow-up screen the high-risk groups of cervical cancer living in high incidence areas. Methods Ｔhe target population of women (30~59 years old) were firstly screened using vinegar (VIA) and iodine (VILI) staining methods, and then combined with an electronic colposcopy and further pathological examination for final definitive diagnosis. Results 5595 people were screened by VIA and VILI and 3676of them were checked by electronic colposcopy and further pathological examination. The pathology results confirmed that there were 189 cases of CINⅠ, 25 cases of CINⅡ, 19 cases of in situ CIN Ⅲ/carcinoma and 8 cases of invasive cervical. Conclusion The accurate rate of cervical cancer screening using iodine and vinegar staining methods was 41.6%and 64.0%,respectively. The iodine and vinegar staining methods combined with electronic colposcopy and biopsy under positioning pathological examination for cervical cancer can greatly enhance the accurate rate of precancerous lesions and early cervical cancer diagnosis.