survivin蛋白在人非小细胞肺癌中的表达及其与bcl-2、HSP90蛋白表达和细胞凋亡的关系

doi:10.3971/j.issn.1000-8578.2009.10.011

survivin蛋白在人非小细胞肺癌中的表达及其与bcl-2、HSP90蛋白表达和细胞凋亡的关系

Expression of survivin Protein in Non-small Cell Lung Cancer and Relationship with bcl-2，HSP90 Protein and Apoptosis

摘要

摘要: 目的探讨survivin蛋白在非小细胞肺癌(NSCLC)中的表达，以及与bcl-2、HSP90蛋白表达及细胞凋亡的相互关系。方法应用Western blot法检测80例NSCLC肿瘤组织、20例肺良性病变组织中survivin、HSP90、bcl-2蛋白表达情况，用TUNEL法检测细胞凋亡的情况，并将结果进行相关分析。结果 survivin蛋白在肺良性病变组织中不表达，在71.25％ (57/80)的NSCLC组织中有表达；survivin表达水平与细胞凋亡指数呈负相关。肺癌组织bcl-2蛋白阳性表达率56.25％(45/80)明显高于肺良性病变组织的10.00％(2/20)，且癌组织中表达水平较肺良性病变组织高，两者比较差异有统计学意义；肺癌组织中HSP90蛋白表达水平明显高于肺良性病变组织。肺癌组织中HSP90、bcl-2蛋白与survivin蛋白表达显著相关(P<0.05)。结论 survivin蛋白在肺癌组织中表达上调，提示该基因对NSCLC的发生发展起重要作用。survivin蛋白表达增高与细胞凋亡减少密切相关，survivin有望成为肺癌基因治疗的新靶点；survivin蛋白的表达与HSP90、bcl-2蛋白的表达均呈正相关，三者在肺癌的发生中可能起协同作用。

Abstract: Objective To investigate the relationship between the expression of survivin，bcl-2，HSP90 protein and apoptosis in non-small cell lung cancer(NSCLC)． Methods Western blot to detect the expression of survivin，bcl-2，HSP90 protein in 80 cases of NSCLC and 20 cases of benign lung lesion. TUNEL assay was applied to detect cell apoptosis. Results Survivin was expressed in 57 of 80 (71.25%) cases of NSCLC and in 0 of 20 (0%) cases of benign lung lesion . There was negative correlation between the expression of survivin and apoptosis index. The positive rate of bcl-2 in lung carcinoma was 56.25% (45/80), much higher than that in benign lung lesion (10%) and the level of expression of bcl-2 and HSP90 in NSCLC was higher than that in benign lung lesion. There was significant correlation among the expression of survivin,HSP90 and bcl-2 in NSCLC． Conclusion survivin protein overexpression plays an important role in the occurrence and progression of NSCLC．There was significant correlation between the expression of survivin and the reducing of cell apoptosis.survivin may be identified as a new therapeutic target．The expression of survivin，bcl-2 and HSP90 was positive correlative, they cooperated in the occurrence and progress of NSCLC．

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