Abstract: Objective To study the effects of SFN on A549 cell lines through analyzing inhibitory rate and the CYR61 expression of A549 cell. Methods The inhibition of SFN on A549 was detected by MTT and CYR61 expression level intervened by SFN was identified by real-time PCR. Results MTT results indicated that SFN had a restrain effect in a dose-dependent manner when the concentration exceeded 40 μM. For the inhibitory ratio, it was in a time-dependent among the dose groups of 40 μM, 80 μM, 100 μM, and 160 μM. A549 cells treated with SFN for 32 hour shown crimped under the microscope and high refraction quantity cell configuration at 25 μM; the amount of crimpled cells was increased at 50 μM; part of the cells were floating and became to fragments at 75 μM. The expression of CYR61 was higher in the group of 25 μM and 50 μM. There was no significant difference between 25 μM and 50 μM group. The expression of CYR61 was lower in 75 μM group than that in 50 μM group. Conclusion SFN inhibited growth of A549 cells in a does-dependent manner and promoted the expression of CYR61. So distilled SFN may be a preventive factor for lung cancer development.