Abstract: Objective To investigate the effect of mfn2 gene on expresssion of RECK gene in breast carcinoma cell (MCF-7). Methods The pEGFP-mfn2 was transfected to MCF-7 with lipofectamine2000, the mRNA levels of mfn2 and RECK were detected by RT-PCR ; Western blot analysis was used to detect the protein expression of mfn2 and RECK gene. Results After transfected by pEGFP-mfn2, the stable expression of Mitofusin-2 gene was detected in MCF-7 cells, the expression of RECK mRNA and protein were significantly activated in transfected Mitofusin-2 gene group, but not detected in transfected pEGFP and non-transfected cells. Conclusion The mfn2 gene can activate the expression of RECK in MCF-7.