Abstract: Objective To investigate the expression of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor C (VEGF-C) in colon carcinoma, and to evaluate its clinical implication. Methods Immunohistochemistry (SP) was used to detect the expression of HIF-1α and VEGF-C in 45 tissues of colon carcinoma. The relationship between the expression of HIF-1α and VEGF-C and the clinicopathological parameters was analyzed. Results In 45 cases,the positive rate of HIF-1α was 66.7%. And the positive rate of HIF-1α in colon cancer tissue expression with lymph node metastasis was higher than that without lymph node metastasis (84.2% vs. 53.8％). The positive rate of VEGF-C was 53.3% in colon carcinoma. The positive rate of VEGF-C expression with lymph node metastasis and that without lymph node metastasis was 73.7% and 38.5%respectively. The expression of HIF-1α and VEGF-C was closely correlated with lymph node metastasis and the depth of tumor invasion，but no relationship with tumor size, sex and age. The positive expression of HIF-1α was consistent with the positive expression of VEGF-C. Conclusion The expression of HIF-1α and VEGF-C is correlated with lymph node metastasis in colon carcinoma.