恶性肿瘤患者外周血CD146＋T淋巴细胞的检测及其临床意义
Clinical Significance of Measuring CD146+ Activated T Lymphocyte in Peripheral Blood of Patients with Malignant Tumor
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摘要: 目的 探讨CD146＋T淋巴细胞在肿瘤患者外周血中的变化及其临床意义。 方法 用流式细胞术检测47例健康对照组和188例肿瘤患者外周血中CD3＋CD146＋T淋巴细胞的数量，并观察其在肿瘤疾病中的变化。 结果 （1）与健康对照组比较，总肿瘤组、肝癌、肺癌组、食管癌、乳腺癌、胃癌、胰腺癌组的CD146＋T/T比例显著升高，差异有统计学意义（P<0.001）。（2）与健康对照组比较，肿瘤组的外周血T细胞绝对数是降低的（P<0.05），而CD146＋T绝对数却是与健康对照相似（P>0.05）；（3）恶性肿瘤患者外周血CD146+ T比例与T细胞绝对计数负相关（n=188，r=-0.297,P<0.001）。（4）肿瘤转移组与未转移组的CD146＋T/T比例的比较未观察到差异有统计学意义（P>0.05）。 结论 肿瘤患者外周血CD146＋T比例增高,可能是机体抗肿瘤免疫调节的一个重要方面。Abstract: Objective To study the changes of cluster of differentiation antigen 146 positive (CD146+) T lymphocyte in peripheral blood of patients with malignant tumor and its clinical significance. Methods CD3＋CD146＋ T lymphocyte in peripheral blood was detected in 47 normal control and 188 patients with malignant tumor by flow cytometry，and the amount and proportion change of CD146＋ T lymphocyte in patients with malignant tumor were observed. Results （1）Compared with normal control, CD146+ activated T lymphocyte percentage(CD146＋T/T) of total tumor group，liver cancer group，lung cancer group，esophageal carcinoma group，breast cancer, gastric cancer and pancreatic cancer group were obviously higher(P<0.001). （2）Compared with normal control,the gross of T lymphocyte in peripheral blood of tumor group was reduced (P<0.001),and there was no difference in number of CD146+ T lymphocyte in peripheral blood similar (P>0.05).（3）There was negative correlation between CD146＋T percentage and T lymphocyte counts in peripheral blood of patients with malignant tumor(n=188，r=-0.297,P<0.001).（4）The percentage of CD146+T(CD146+T/T) was no significant difference between metastasis group and no metastasis group(P>0.05). Conclusion The percentage of CD146+ T lymphocyte in patients with tumor was increased， the changes may be an important aspect on antitumor immunoregulatory in malignant tumor patients.
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