Abstract: Objective To investigate the effect on proliferation and adhesion of vWF in human gastric cancer cell line BGC-823 in vitro, and to clarify its probable mechanism. Methods Human gastric cancer cell line BGC-823 cultured in vitro was treated with vWF antibody (vWF Ab).The effect of vWF on the proliferation and adhesion of BGC-823 in vitro was detected respectively by MTT colorimetric assay. The cell morphological changes were observed with inverted microscope. Results vWF Ab inhibited the proliferation of BGC-823 in a time-and concentration-dependent manner. Significant cytotoxicity of vWF Ab on BGC-823 cells was observed by cell morphology. Compared with the control group, the adhesion ability of the BGC-823 cells was decreased significantly (P<0.01) after treatment with vWF antibody. Conclusion vWF has significant effect on the proliferation of BGC-823 cells and the cell morphological change in vitro, vWF takes an important part in the progress of adhesion on the BGC-823 cells.