抵抗素13肽对人乳腺癌细胞系MCF-7增殖和凋亡的影响

doi:10.3971/j.issn.1000-8578.1840

抵抗素13肽对人乳腺癌细胞系MCF-7增殖和凋亡的影响

Effect of Resistin-13-peptides on Proliferation and Apoptosis of MCF-7 Cells in vitro

摘要

摘要: 目的研究人工合成抵抗素13肽对人乳腺癌细胞系MCF-7增殖和凋亡的影响。方法根据人抵抗素cDNA编码的氨基酸序列合成抵抗素分子22—34位的13个氨基酸的多肽（CSMEEAINERIQE）。采用MTT法检测细胞的增殖活性，使用流式细胞仪检测细胞凋亡。结果50、500、5000和10000ng／ml抵抗素13肽均能抑制MCF-7细胞生长，抑制率为46％到92％，与对照组相比差异有统计学意义（P〈0．01）。诱导凋亡，四个浓度诱导的细胞凋亡率分别为6．7％、6.9％、7.5％和9．8％，与对照组0．6％的凋亡率比较P〈0．01。结论抵抗素13肽可以抑制乳腺癌细胞系MCF-7的增殖，促进细胞凋亡。

Abstract: Objective 　To investigate the potential effect s of resistin2132peptides on the growth and apopto2 sis in human breast carcinoma cells, MCF27. Methods 　Basing on the deduced amino2acide sequence f rom the cDNA, a peptide of human resistin molecule, consisting of 13 amino2acide (CSMEEAINERIQE) was synthesized. 32(4, 52Dimethyl222thiazolyl)2 2, 52diphenyl22 H2tet razolium bromide (MTT) assay was used to assess the proliferation effect s of resistin2132peptides. The apoptosis was investigated by the flow cy2 tometer, FCM. Results 　With the Resistin2132peptides t reatment of 50 、500 、5 000 and 10 000 ng/ ml, the inhibitory rate was 46 % to 92 %. The discrepancy between test and cont rol group s is statistically signifi2 cant ( P < 0. 01) . The apoptotic rate was 6. 7 %、6. 9 %、7. 5 % and 9. 8 % respectively. And the rate of cont rol group was 0. 6 % ( P < 0. 01) . Conclusion 　Resistin2132peptides can inhibit the proliferation of the cell proliferation and promote the apoptosis of the cells.

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