荧光定量聚合酶链反应检测EB病毒感染
Fluorescence quantitative PCR detected Infection of Epstein-Barr virus
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摘要: 目的 建立检测EBV感染的新方法并观察EBV与鼻咽癌的关系。方法 鼻咽部活检组织和石蜡包埋标本 6 6例, 采用荧光定量聚合酶链反应 (FQ PCR)和原位杂交 (ISH)两种方法检测EBV, 其检测结果与病理诊断对照。结果 FQ PCR检测EBV DNA阴性 5 2例, 其中 5 1例为炎性病变, 另 1例为腺癌 ;EBV DNA阳性 14例, 其中 13例为鳞状细胞癌, 另 1例虽为炎症, 但部分上皮细胞呈不典型增生。ISH检测EBV阴性 4 9例, 其中 4 7例为炎性病变, 2例为癌。EBV阳性 17例, 其中 12例为癌, 5例为炎性病变。FQ PCR和ISH检测EBV结果, 经卡方检测差异无显著性 (P >0 .0 5 )。结论 EBV感染与鼻咽癌有高度相关性。FQ PCR检测EBV具有方法简单、快速、定量准确等优点Abstract: Objective To establish a novel method for detecting infection of Epstein-Barr Virus(EBV)and to observe relation between EBV and nasopharyngeal carcinoma.Method Real time fluorescence quantitative PCR(FQ-PCR) and in situ hybridization(ISH) method were used to detect infection of EBV in 66 cases specimens of nasopharyngeal biopsy and embeded in paraffin,the results were compared with pathologic diagnosis. Results FQ-PCR detected EBV-DNA negative 52 cases,51 cases was inflammatory disease,1 cases was ade...
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