端粒酶基因和抗凋亡基因bcl-2在肺组织中的表达研究
Study on expression of telomerase gene and auti-apoptosis gene in human lung cancer
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摘要: 目的 研究肺癌端粒酶基因和bcl 2的关系, 以期阐明端粒酶基因及抗凋亡基因对端粒酶的调控机理。方法 采用TRAP、RT PCR及LSAB的方法研究了肺癌端粒酶活性、hTR、hTERT及bcl 2的表达。结果 82 .3%的肺癌组织表达端粒酶阳性, 38例癌组织中, 34例表达hTR (89.4 % ), 36例表达hTERT(94 .7% )。而在相应的癌旁组织中, 34例表达hTR, 但只有 3例表达hTERT(7.8% )。结论 肺癌组织中具有较高的端粒酶活性, 在肿瘤组织和正常组织中hTERT的表达有显著的差异 (P <0 .0 5 )。hTR在正常组织和肿瘤组织均有较高的表达, bcl 2的表达与端粒酶基因有着较高的相关性。Abstract: Objective To investigate the relationship between the telomerase gene and anti-apoptosis gene bcl-2 in lung cancer . Methods Expression of the telomerase gene,Bcl-2 gene were detected by RT-PCR and immunohistochemical techniques respectively. And telomerase activity was examined by TRAP. Results In 38 lung cancer tissues, telomerase activity, hTR and hTERT were expressed in 82.3%,89.4% and 94.7% respectively. Most normal lung tissues expressed hTR(89.4%) and hTERT was detected in only 3 of 38 non-neop...
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