23-羟基白桦酸抑制血管内皮细胞增殖和诱导凋亡的实验

doi:10.3971/j.issn.1000-8578.1696

23-羟基白桦酸抑制血管内皮细胞增殖和诱导凋亡的实验

Proliferation Inhibition and Apoptosis Induction of HMEC Cells by 23 -HBA

摘要

摘要: 目的研究23-羟基白桦酸（23-hydroxy betulinic acid，23-HBA）对人血管内皮细胞（human microcapillary endothelial cells，HMEC）增殖及凋亡的影响。方法采用SRB法测定23-HBA对HMEC增殖的抑制率，并用流式细胞术测定23-HBA对HMEC周期及凋亡的影响。结果 23-HBA可明显抑制HMEC的体外增殖，IC50为40．44μg／ml。流式细胞术测定，HMEC出现典型的凋亡峰，其凋亡百分率随着药物浓度的提高而明显升高，与对照组相比，差别有统计学意义。HMEC的S期细胞含量明显上升，而G2／M期细胞含量下降。结论 23-HBA具有明显抑制血管内皮细胞增殖，诱导细胞凋亡的作用。

Abstract: Objective 　To study the proliferation-inhibitng and apoptosis-inducing effect s of 23-hydroxy betulinic acid ( 23-HBA ) on human microcapillary endothelial cells ( HMEC) . Methods 　The effect of 23-HBA on in vitro proliferation of HMEC was examined by SRB assay. The effect of 23-HBA on the cell cycle and apoptosis were analyzed by flow cytometry. Results 　The proliferation of HMEC was inhibited significantly by 23-HBA with IC50 being 40. 44μg/ ml. Apoptosis peak was also shown by flow cytometry. The percentage of cell apoptosis increased with the increase of the drug concent ration, and the increase rate was significantly higher than that of the control cell group. The content of cells increased in Sphase and decreased in G₂ / M phase. Conclusion 　23-HBA can inhibit HMEC proliferation and induce apoptosis, could be developed as an antiangiogenic drug.

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