~(60)Co γ射线照射后血管内皮细胞CD54表达变化及PDTC的干预作用
Expression of CD54 in the Blood Vessel Endothelial Cells Post ~(60)Co γ Ray Irradiation and Intervention Effect of PDTC
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摘要: 目的 研究血管内皮细胞在受60 Coγ射线照射后粘附分子CD5 4的表达与单核样细胞在血管内皮细胞上粘附变化的关系, 观测抗氧化剂PDTC预处理对CD5 4表达及白细胞粘附的影响。方法 培养的ECV30 4细胞接受 16Gy60 Coγ放射线照射后不同时点, 检测视黄酸诱导的单核样HL 6 0细胞在内皮细胞上的粘附数量, 内皮细胞CD5 4mRNA及蛋白表达, 核转录因子κBDNA结合活性以及抗氧化剂PDTC预处理对上述指标的影响。结果 照射后 8h, HL 6 0细胞粘附数较对照显著上升, 2 4h时较对照增加 77%。受照后 2h, CD5 4mRNA水平较对照增加 36 %, 2 4h增加 182 %。照射后 4～ 32h, CD5 4蛋白表达较对照增加 6 6 %～ 2 6 8%。NF κB DNA结合活性在照射后 2h达峰值, 较对照增高 97%。PDTC预作用后 2h, NF κB DNA结合活性较未处理组下降了 4 4 %, 作用后 4h, CD5 4mRNA表达量下降 35 %, 同时HL 6 0细胞粘附数减少 2 6 %。结论 CD5 4表达上调是60 Coγ射线照射引起单核细胞在内皮细胞上...Abstract: Objective To elucidate the correlation between CD54 expressions in the blood vessel endothelial cells irradiated with 60Co γ ray and adhesion of monocyte, such as HL-60, on the irradiated cells, and to study the effect of antioxidant PDUC on this process. Methods At 2h, 4h, 8h and 24h after ECV304 cells were exposed to 60Co γ ray, the RA-induced HL-60 cells adhered to the irradiated endothelial cells were counted, expressions of CD54 mRNA and protein were measured by RT-PCR and FCM, respectively,...
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