三氧化二砷经肝动脉栓塞化疗对兔肝移植瘤的作用
Effects of Arsenic Trioxide by Transcatheter Arterial Chemoembolization on Rabbits with Hepatic Vx-2 Carcinoma
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摘要: 目的 探讨As2 O3 经肝动脉栓塞化疗对肝移植瘤的作用。方法 采用实验组 1(生理盐水 )、实验组 2 (碘化油 )、实验组 3(As2 O3 +碘化油 )经肝动脉注射治疗肝移植瘤一次, 实验组 4 (As2 O3 )经肝动脉插管灌注药物 (连续 7天 )。观察肿瘤平均瘤重、平均瘤重抑制率、肿瘤细胞形态学变化, 检测bcl 2 /bax基因表达和VEGF表达。结果 实验组平均瘤重分别为 16 .0 8± 2 .6 9g、3.4 4± 0 .84 g、1.5 2± 0 .2 6 g和4 .87± 0 .73g, 平均瘤重抑制率分别为 78.6 1%、90 .5 5 %和 6 9.71%, 实验组 3移植瘤瘤重低于其他实验组 (P <0 .0 5 )。实验组 3、4移植瘤组织bax基因表达显著上调、bcl 2基因表达显著下调 (P <0 .0 5 )、VEGF表达下调 (P <0 .0 5 ) ;电镜观察实验组 3、4均有典型的瘤细胞凋亡改变。结论 As2 O3 经肝动脉栓塞化疗对兔Vx 2肝移植瘤有显著抑瘤作用, 碘化油能增强As2 O3 对肝移植瘤的抑制作用, 两药具有协同作用。Abstract: Objective To evaluate the anticancer effect of arsenic trioxide(As 2O 3) by transcatheter arterial chemoembolization (TACE) on rabbits with hepatic Vx-2 carcinoma. Methods Forty New Zealand rabbits with hepatic Vx-2 carcinoma were managed with normal saline (experimental group 1), lipiodol and normal saline (experimental group 2), As 2O 3 and lipiodol (experimental group 3) by hepatic artery infusion respectively, whereas experimental group 4 was treated with As 2O 3 by TAI for 7 days consecutivel...
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