奥曲肽对胃癌细胞株SGC7901细胞生长抑制作用及其机制的探讨

Effect and Mechanism of Octreotide on the Viability of Gastric Cancer Cell Line SGC7901 Cells

摘要: 目的　研究生长抑素类似物奥曲肽对胃癌细胞株SGC790 1细胞的增殖、蛋白激酶B及端粒酶活性的影响。方法　应用奥曲肽作用于胃癌细胞株SGC790 1细胞不同时间后, 检测其对体外培养胃癌细胞的抑制率, 胃癌细胞蛋白激酶B(Akt/PKB)及端粒酶的活性。结果　奥曲肽对胃癌细胞增殖有明显抑制作用, 呈剂量依赖性。同时能显著抑制胃癌细胞Akt/PKB的活性和端粒酶的活性 (12, 2 4, 4 8h均P <0 .0 1)。结论　生长抑素对胃癌细胞株SGC790 1细胞增殖有抑制作用, 其机制与抑制Akt/PKB及端粒酶活性有关。

Abstract: Objective To study the effect of octreotide on cell viability, protein kinase B and telomerase activity in human gastric cancer cell line SGC7901. Methods After incubation for 0,12,24,48 hours in different concentration of octreotide, MTT assay was used to determine the cell viability. Akt/PKB and telomerase activities were respectively detected. Results SGC7901 cells exhibited a dose-dependent inhibition of growth. The Akt/PKB and telomerase activity of SGC7901 cells was significantly inhibited by oct...