肠复康对人大肠癌HT-29细胞增殖的影响及作用机制研究

Influence and Mechanism of Changfukang on Humam Large Intestine Cancer HT-29 Cell Proliferation

摘要: 目的　研究中药复方肠复康对人大肠癌细胞增殖的影响及探讨其作用机制。方法　建立人大肠癌HT 2 9裸鼠移植瘤模型。设立肠复康低、中、高剂量组和空白组。给药 8周后比较瘤重、抑瘤率及病理变化。免疫组化染色结合图像分析系统半定量检测移植瘤Ki 6 7阳性细胞数和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)的积分光密度。结果　肠复康低、中、高剂量组抑瘤率分别为33.13%、4 7.2 6 %、6 6 .0 1%。肠复康各组移植瘤细胞核数量较少, 细胞及细胞核形态更加规则。肠复康各组Ki 6 7阳性细胞数及VEGF表达均低于空白组。结论　肠复康具有抗人大肠癌HT 2 9裸鼠移植瘤细胞增殖的作用, 其机理可能与促进癌细胞分化及抑制VEGF的表达有关。

Abstract: Objective To investigate the influence of Chinese Compound Recipe Changfukang on humam large intestine cancer cell proliferation and explore its mechanism. Methods Model nude mouse of transplantation tumor of human large intestine cancer HT-29 were established. The low, medium and high dose groups of Changfukang were set up.Eight weeks after medication, the tumor weight, inhibition rates and pathologic changes of each group were compared. The number of positive Ki-67 cells and the inttegra optical densit...