CYP2C9、GSTM1基因多态性与肺癌易感性的关系
Relationship between CYP2C9 and GST M1 Genetic Polymorphism and Lung Cancer Susceptibility
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摘要: 目的探讨细胞色素P4502C9(CYP2C9)基因、谷胱甘肽硫转移酶M1(GST M1)基因多态性与肺癌易感性的关系。方法用PCR-RFLP法分析56例肺癌(简称肺癌组)和42例健康对照组NsiⅠ识别的CYP2C9基因型;用PCR法分析其GST M1基因型。结果突变型CYP2C9*3型基因发生频率在肺癌组中为8.93%,高于对照组的4.76%,其差异无显著性(P>0.05)。肺癌组GST M1基因缺失型〔GST M1(-)〕发生率为71.43%,高于对照组的45.24%,其差异有显著性(P<0.01);GST M1(-)型与肺癌呈高度联系强度,OR=3.09(95%CI=1.32~6.94);GST(-)基因型在吸烟的肺癌组(81.08%)与对照组(52.18%)之间的频率差异有显著性(P<0.05)。结论突变型CYP2C9*3型基因与肺癌无显著性联系;GST M1(-)基因型是肺癌发生的遗传易患性因素;吸烟可显著提高GST(-)基因型个体患肺癌的危险性。Abstract: Objective To evaluate the relationship between CYP2C9, GST M1 genetic polymorlism and susceptibility of lung cancer. Methods The polymerase chain reaction-restriction fragment length polymorphlism (PCR-RFLP) was used to detect CYP2C9 genotype in 56 lung cancer cases and 42 controls. The PCR was also used to detect GST M1 genotype in the two groups. Results The frequencies of mutation genotype of CYP2C9*3 was not significant difference between lung cancer cases and controls.However, in the lung cancer cases, ...
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