肝癌患者PBMC中TH1/TH2类细胞因子mRNA表达
Expression of TH1/TH2 Cytokines mRNA of PBMC in Hepatocarcinoma Patients
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摘要: 目的探讨TH1类细胞因子IL-2、IFN-γ及TH2类细胞因子IL-4mRNA在Ⅰ期和Ⅱ期肝癌患者外周血单个核细胞(PBMC)中的表达模式,并观察IL-2、IFN-γmRNA在手术前后的表达变化。方法采用逆转录聚合酶链反应(RT-PCR)方法测定8例Ⅰ期和10例Ⅱ期原发性肝癌患者手术前后及15例健康人外周血中IL-2、IFN-γ的表达,并用凝胶图像分析系统进行图像分析。结果在18例肝癌患者中,IL-2、IFN-γmRNA的表达率分别为16.7%、5.6%,低于IL-4的表达率88.9%(P<0.05),呈现明显的TH2偏移状态,且IL-2、IFN-γ的表达强度在0.10~0.11之间,也远低于IL-4的0.21~0.22;在8例Ⅰ期肝癌患者中,术前有25.0%(2/8)表达IL-2mRNA,12.5%(1/8)表达IFN-γ,高于对照组的13.3%(2/15)、5.6%(1/15);手术治疗后两者的表达率分别是50.0%、37.5%,高于术前组;术前组及正常对照组两种细胞因子mRNA表达强度(0.10~0.12)明显低于术后组的0.20~0.22(P<0.05)。另外,10例Ⅱ期患者手术前后术均无IFN-γm...Abstract: Objective To explore the expression pattern of TH1 type cytokines IL-2、IFN-γmRNA and TH2 type cytokine IL-4 mRNAin peripheral blood mononuclear cells (PBMCs) of primary hepatic cancer(PHC) patients, and observe the different expression of IL-2 and IFN-γmRNA before and after the tumor resection. Methods The expression of IL-2 、IL-4 and IFN-γmRNA of 18 patients and 15 normal volunteers in PBMC was detected by RT-PCR method and the results were analyzed by gel image analysis system. Results The expression of IL-...
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