应用基因芯片技术筛选卵巢癌紫杉醇耐药差异表达基因
Screening Differential Expression of Taxol-resistance Related Gene of Ovarian Carcinnoma by cDNA Microarray
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摘要: 目的应用基因芯片技术研究人卵巢癌紫杉醇耐药细胞株OC3/Tax300与其亲本细胞OC3(敏感株)之间的基因表达谱差异,筛选耐药相关基因,探讨基因表达谱差异与卵巢癌肿瘤耐药之间相关性。方法分别提取C3/Tax300与OC3细胞的总RNA并纯化mRNA;将等量的mRNA逆转录,以Cy5和Cy3标记的cDNA做探针,在Biostar H140S基因表达谱芯片上进行杂交。扫描芯片荧光信号图像,用基因图像分析软件对扫描图像进行数字化处理和分析。结果共筛选出显著表达差异基因134条,其中117种基因表达水平下调,17种基因表达上调,主要涉及细胞信号蛋白和传递蛋白,蛋白翻译合成类以及细胞骨架和运动、离子通道和运输蛋白、代谢、发育等14大类相关基因。下调基因主要为EBNA-3、COP9、StIP1,上调的基因主要是JAK2、HSPS、NADH等。结论这些基因表达差异与卵巢癌化疗耐药有关。Abstract: Objective To study the gene differential expression of taxol resistance cell line OC3/Tax300 of ovarian carcinnoma compared with other parent cell OC3,screen drug-resistance related gene by cDNA microarray, and discuss the reiationship between gene expression difference and drug-resistance. Methods The cDNA retro-transcribed from equal quantity mRNA derived from OC3/Tax300 and OC3 which were labeled with Cy5 and Cy3 fluorescence as probe respectively. The mixed probe were hybridized with BiostarH140S gene ex...
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