Abstract: Objective 　To study the effect elements about different organic micro-environment to and investigate the relationship between MMPs, TGF-β1 and transplant-tumor's biological deed. Methods 　The staining with HE method is conducted to investigate the presentation of different transplant-tumor's biological deed. Immunohistochemistry is used to detect the expression of MMP-2, MMP-9, TGF-β1 in two tumor organ respectively. Results 　①The biological deeds of identical transplant-tumor′s from different body have significant difference in different organic micor-envionment . subcutaneous metastaic carcinoma presents expensive grouth ; Belly metastaic carcinoma presents infilt rative grouth. ②The expression of MMP-2 and MMP-9 : Subcutaneous metastaic carcinoma is negative ; belly metastaic carcinoma is positive. ③The expression of TGF-β₁: In subcutaneons metastaic carcinoma, TGF-β₁ is expressed in interstitial tissue. In belly metastaic carcinoma, TGF-β₁ is expressed in cytoplasm. Conclusion 　Different organicmicro-environment will affect the metastasis and infilt ration of identical transplant-tumor. The expression of MMP-2, MMP-9 and TGF-β₁ is one of it s elements.