实时定量PCR分析CML患者胸腺近期输出功能

Recent Thymic Output Function in Patients with Chronic Myelogenous Leukemia by Real Time PCR Analysis

摘要: 目的　了解慢性粒细胞白血病 (CML)患者CD3+ 、CD4 + 和CD8+ 细胞中T细胞受体重排删除DNA环 (TRECs)的含量, 从而推测CML患者的胸腺近期输出功能。方法　利用实时定量PCR(Taq Man)方法检测 8例CML患者外周血单个核细胞、3例CML患者CD4 + 和CD8+ 细胞中TRECs的水平。并根据外周血中CD3阳性率计算CD3+ 细胞中TRECs水平。 9例正常人外周血作为对照。结果　CML患者中TRECs含量为 0 .1± 0 .2 2 / 10 0 0T细胞, 明显低于正常人TRECs水平 (6 .84± 4 .71/ 10 0 0T细胞, P <0 .0 1)。其中 5例患者外周血单个核细胞中未能检测到TRECs, 经分选CD4 + 和CD8+ 细胞后, 2例可以检测到低水平的TRECs。结论　本研究首先报道了CML患者TRECs水平情况, 结果显示其胸腺近期输出功能明显降低

Abstract: Objective To quantitative analysis the T cell receptor excision DNA circles (TRECs) in CD3 +, CD4 + or CD8 + T cells, evaluating the thymic recent output function in patients with chronic myelogenous leukemia (CML). Methods Quantitative detection of T cell receptor excision DNA circles (TRECs) in DNA of peripheral blood mononuclear cells from 8 cases with CML, CD4 + or CD8 + cells from 3 cases with CML were preformed by real time PCR (TaqMan) analysis. And the TRECs number was related to the numbe...