X刀治疗大鼠C6脑胶质瘤诱发凋亡及抑癌基因p16蛋白的表达

The induced apoptosis and the p16 Gene s expression in treating rat C6 glioma with X-knife

摘要: 目的　为了探讨经X刀照射诱导C6脑胶质瘤凋亡及其抑癌基因p16蛋白的表达。方法　建立大鼠C6脑胶质瘤模型, 经X刀照射后, 采用TUNEL法检测细胞凋亡, 免疫组化SABC方法检测抑癌基因p16蛋白表达的变化。结果　治疗组凋亡阳性细胞数明显高于对照组 (P <0 .0 5 ) ;p16基因在蛋白水平表达率也高于对照组 (P <0 .0 5 )。结论　X线可诱导C6脑胶质瘤细胞发生凋亡, 抑癌基因 p16在辐射诱导凋亡中起重要的作用。

Abstract: Objective To investigate the apoptosis of C6 glioma cell and the p16 gene s protein expression after X-knife radiation. Methods By establishing a rat C6 glioma model,after X-knife, TUNEL and immunohistochemistry technique (SABC methods) was employed to inspect the apoptotic cells and the p16 gene s protein expression respectively. Results X-radiation can induce apoptosis,the apoptosis and the p16 gene s protein expression in the treatment group is higher than that in control group ( P < 0.05 ). Concl...