survivin 反义核酸增强PANC-1 细胞对5-Fu敏感性的研究
Antisense RNA of survivin Enhances the Sensitivity of PANC-1 Cell to 5-Fu
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摘要: 目的 研究survivin反义核酸诱导胰腺癌细胞株PANC-1的凋亡和增强对5-Fu敏感性的影响。方法 我们应用构建的survivin反义核酸真核表达栽体电转染PAND-1细胞，RT-PCR检测survivin mRNA表达，细胞计数和MTT实验测定转染后细胞对5-Fu敏感性，流式细胞仪检测细胞凋亡。结果 survivin反叉核酸可抑制PANC-1细胞中survivin mRNA的表达，并能抑制PANC-1细胞生长，提高PANC-1对5-Fu敏感性，流式细胞仪检测凋亡率明显增加。结论 survivin反义核酸能增强5-Fu诱导的PANC-1细胞凋亡，联合survivin反义核酸和5-Fu可以改善胰腺癌的治疗效果。Abstract: Objective To explore the apoptosis of PANC-1 cells induced by antisense RNA of survivin and the increasing sensitivity effect to 5-Fu. Methods We t ransferred the const ruction recombination antisense RNA of survivin of eukaryotic vector into PANC-1 cells by elect roporation. Expression of survivin mRNA was determined by RT-PCR. Cell Counting and MTT assay were performed to evaluate the sensibility of t ransferred cells to 5-Fu. The rate of apoptosis was tested by FCM. Results Transfection of the antisense RNA of survivin can inhibit the expression of survivin mRNA and inhibit the growth of PANC-1 Cells. The sensitivity of PANC-1 cells to 5-Fu was enhanced after t ransfection . The rate of apoptosis increased af ter antisense RNA of survivin was transferred into PANC-1 cells. Conclusion Antisense RNA of survivin could enhance 5-Fu-induced apoptosis in PANC-1. Combination of Antisense RNA of survivin and 5-Fu could improve the treatment effect of pancreatic cancer.
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