应用组织微阵列技术研究胃癌hMSH2 和p16<sup>INK4a</sup>的表达意义

应用组织微阵列技术研究胃癌hMSH2 和p16^INK4a的表达意义

摘要: 目的探讨错配修复基因hMSH2和抑癌基因p16^INK4a在胃癌组织中表达的意义。方法构建胃癌组织微阵列(TMA)，采用免疫组化法测定hMSH2和p16^INK4a的表达。结果在胃癌组织中，hMSH2和p16^INK4a的过表达呈正相关(r=0．235，P=0．0134)，与有无淋巴结转移及转移程度、生存时间无关。结论 hMSH2和p16^INK4a的过表达呈正相关，两者均不能作为预测有否淋巴结转移、转移程度和预后的分子标志物。

Abstract: Objective 　This study was to investigate the expression meaning of hMSH2 and p16^INK4a in gastric cancer. Methods 　Gastric cancer tissue microarray(TMA) was formed and tested expression of hMSH2 and 16^INK4a by immunohistochemical methods. Results 　In gastric cancer, the overexpression rates of hMSH2 and 16^INK4ashowed a positive correlation ( r = 0. 23507, P = 0. 0134), which were not related to lymph node metastasis, UICC N stage and prognosis. Conclusion 　the overexpression rates of hMSH2 and 16^INK4a showed a positive correlation and both were not as molecule mark to predict lymph node metastasis, UICCN stage and prognosis.