HSV-tkGCV系统联合重组人TNF-α治疗脑胶质瘤的离体实验研究
Combination Therapy for Glioma with HSV-tk/GCV System and Recombinant Human Tumor Necrosis Factor-alpha
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摘要: 目的 探讨HSV tk GCV系统联合rhTNF α抗脑胶质瘤的有效性。方法 利用重组HSV tk逆转录病毒载体转染大鼠C6细胞得到HSV tk阳性细胞, C6 GCV、C6+tk GCV、C6+rhTNF α和C6+tk GCV +rhTNF α4组在体外分别给予GCV或 和rhTNF α处理, 用MTT法测量细胞存活率, 比较各组肿瘤细胞存活率。卡方检验作显著性检测。结果 未加任何抗癌因子处理的C6细胞组肿瘤细胞生长旺盛, 细胞形态无明显改变 ;各处理组的肿瘤细胞 2～ 3天后开始逐渐出现细胞突触消失, 细胞变圆, 胞体皱缩, 核仁固缩、裂解、甚至消失, 继而逐渐死亡。联合应用HSV tk GCV +rhTN α组的肿瘤细胞存活率比单纯应用HSV tk GCV组或rhTNF α组明显低 (P <0 0 5)。结论 :联合应用HSV tk GCV系统和rhTNF α的杀癌细胞效果优于单一使用HSV tk GCV系统或者rhTNF α。Abstract: Objective To investigate the synergistic anti-glioma effects of HSV-tk/GCV system and Recombinant Human Tumor Necrosis Factor-alpha(rhTNF-α)in vitro. Methods The HSV-tk gene was transferred into C6 cells to obtain C6 tk + cells.The test was divided into four groups:C6 cells group、C6tk/GCV group、C6+rhTNF-α group and C6+tk/GCV+rhTNF-α group.Each group was treated with GCV or/and rhTNF-α.The cells survival rates of every group were determined by MTT technique. Results The tumor cells without any anti-glioma fac...
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