温热顺铂对肝癌细胞的杀伤效应及其诱导凋亡的研究
Studies on the effect of cell killing and relationship between heated-CDDP and induction of apoptosis in Hca-F
-
摘要: 目的 探讨温热 CDDP对 Hca-F细胞的生长抑制、温度与药效的关系以及诱导凋亡的作用。方法 采用MTT法,透射电镜,DNA电泳以及流式细胞术分析法。结果CDDP对Hca-F细胞有明显的细胞毒性,其IC50值为0.57μg/ml。CDDP合并高温(>41℃)加热明显提高了CDDP的杀伤力,二者之间存在着协同增敏作用。其作用强度呈现对药物浓度、作用时间与加热温度的正相关性,且在一定浓度范围内以谤导肿瘤细胞凋亡的发生为主。结论 加热(>41℃)顺铂提高了顺铂的细胞毒性。诱导细胞凋亡的发生是其抗肿瘤作用的主要机制之一。Abstract: Objective To determine the effect of inhibition of cell growth of hyperthermia in combination with CDDP on Hca-F cell lines, and the relationship between temperature and medical effect as well as re- lationship between heated-DDP and induction of apoptosis in Hca-F. Methods Observing the relationship between temperature and effect of CDDP by typanblue assay and transmission electron microscopic tech- nique and flow cytometry as well as DNA fragmentation. Results Heating-C...