氧化砷诱导结肠癌细胞凋亡及其分子机制

Studying of Molecular Mechanism of Apoptosis of Colorectal Cancer Cell Line Induced by Arsenic Trioxide

摘要: 目的　观察不同浓度As2O3对结肠癌Lovo、LS-174T细胞株生长的影响。方法　0.125～2μ mo1/L的As2O3与结肠癌细胞株Lovo、LS-174T共同孵育，观察不同浓度As2O3作用不同时间对结肠癌细胞的生长抑制作用、细胞形态学改变、DNA的变化。结果　1～2μ mol/L As2O3作用的结肠癌细胞呈凋亡特征性改变：细胞膜不发生破裂，细胞核出现核浓缩、核碎裂和凋亡小体形成；流式细胞仪检测在G1期前出现亚二倍体凋亡峰；细胞DNA抽提琼脂糖凝胶电泳呈凋亡特征性条带。结论　1～2μ mol/L As2O3可诱导结肠癌细胞凋亡，肿瘤细胞凋亡与Fas、Fas-L表达有关。

Abstract: Objective To observe the effects of a wide-range concentration of arsenic trioxide on the growth of colorectal cancer cell lines lovo and LS-174T. Methods Colorectal cancer cells were cultured with 0.125-2 μ mol/L arsenic trioxide for various duration.Cell activity morphologic changes and cell-DNA distribution were studied. Results Colorectal cells cultured with 1～2 μ mol/L As2O3 presented apoptotic features:intact cell membrane,chromatin condenstation,nucleic fragmentation and apoptotic body formation.Flow cytometry analysis showed a sub-G1 cell peak.Agorose eletrophoresis showed marked DNA ladder. Conclusion 1～2 μ mol/L As2O3 can induce apoptosis of colorectal cancer cells and this apoptosis has some relation with the expression of Fas and Fas-L.