钙和维生素D对高脂饮食诱发的小鼠乳腺上皮增生的影响
Effects of dietary calcium and vitamin D on induced epithelial hyperproliferation in mouse mammary gland by a high-fat diet
-
摘要: 本研究目的是观察饮食中添加钙和维生素D对高脂饮食诱发的小鼠乳腺上皮细胞增生的影响。将四周龄小鼠随机分成三组，分别给予对照AIN－76A饲料，高脂低钙低维生素D饲料（试验饲料Ⅰ）和高脂加钙加维生素D饲料（试验饲料Ⅱ）。饲养九周后终止试验，小鼠植入渗透泵灌注溴脱氧尿苷（Brdu）72小时。结果显示试验饲料Ⅰ组的小鼠乳腺终末小导管上皮细胞Brdu标记指数与对照饲料组比较明显升高（P<0.05）。而试验饲料Ⅱ组的BrdU标记指数与对照组相似（P＞0.05）。本实验研究发现进一步证明，高脂饮食能诱发小鼠乳腺小导管上皮细胞的增生，同时也提示饮食中添加钙和维生素D有助于预防高脂饮食的这种不良作用。Abstract: The present study was designed to investigate the effects of added dietary calcium and vitamin D on the development of epithelial cell hyperproliferation induced in the mammary gland of mice by a high-fat diet. Four-week-old C57BL/6J mice were randomly divided into three groups, and given control diet (AIN-76A), high-fat diet with reduced calcium and vitamin D (Experimental diet i) and high-fat diet with addition of calcium and vitamin D (Experimental diet I ), respectively. Nine weeks after the dietary intervention, the animals were implanted with osmotic pumps for 72 hours to infuse bromodeoxyuridine (BrdU). Findings revealed that mice on Experimental diet I had significantly increased (P M 0. 05) BrdU--labeling indices of the terminal ducts of the mammary gland, compared to control diet group i however, BrdU--labeling indices of the terminal ducts were similar (P 0. 05) in the control diet and Experimental diet Ⅱgroups. The present study further demonstrates that a high--fat diet induces hyperproliferation of epithelial cells in the terminal ducts, and also suggests that this effect of the high-fat diet can be prevented by increasing dietary calcium and vitamin D.
-
Key words:
- mammary gland /
- epithelial hyperproliferation /
- calcium /
- vitamin D
-
-
1 Nationl Center for Health Statistics;US Dept. Health Human Services，1990.172:4～60 2 Xue I_X. Newmark HI，I_ipkin M. Mammary gland hyperproliferation and hyperplasia induced in mice by a Western-style diet. Cancer Res，1995.36:596 3 Xue LX. Newmark HI，I_ipkin M. Model of mouse mammary gland hyperproliferation and hyperplasia induced by a Western-style diet. Nutr Cancer. 1996.26:281～287 4 Newmark HI，Lipkin M. Maheswari N. Colonic hyperplasia and hyperproliferation induced by a nutritional stress diet with four components of Westen-style diet. J Natl Cancer Inst. 1990. 82:491～496 5 Newmark HI_. I_ipkin M. Maheswari N. Colonic hyperproliferation induced in rats and mice by nutritional stress diets containing four components of a human Westernstyle diet (series 2). Am J Clin Nutr. 1991.54:209～214s 6 Lipkin M. Newmark HI.Effect of added dietary calcium on colonic epithelial-cell proliferation in subjects at high risk for familial colonic cancer. N Eng J Med. 1985，313: 1381～1384 7 Ad Hoc Committee on standards for nutritional studies.Report of the American Institute of Nutrition Ad Hoc Committee on standards for nutritional studies. J Nutr. 1977. 107:1324～1434 8 Wellings SR. ]ensen HM. On the origin and progression of ductal carcinoma in the human breast.J Natl Cancer Inst.1973. 50;1111～1118 9 Wellings SR. Jensen HM. Marcum RG. An atlas of subgross pathology of the human breast with special reference to possible precancerous lesions. J Natl Cancer Inst，1975，55:231～273 10 Zhang I_. Bird RP. Bruce WR. Proliferative activity of murine mammary epithelium as affected by dietary fat and calcium. Cancer Res. 1987,47:4905～4908 11 Carroll KK. Khor HT. Dietary fat in relation to tumorigenesis. Prog Biochem Pharmacol. 1975.10:308～353 12 Armstrong B, Doll R. Environmental factors and cancer incidence and mortality in different countries, with special reference to dietary practices. Int J Cancer. 1975.15:617～631 13 Welsch CW. House ]I，Herr Bl，et al. Enhancement of mammary carcinogenesis by high levels of dietary fat: A phenomenon dependent on ad libitum feeding. J Natl Cancer Inst. 1990. 82:1615～1620 14 Hopkins GJ，Carroll KK. Relationship between amount and type of dietary fat in promotion of mammary carcinogenesis induced by 7. 12-dimethylbenz (a ) anthracene. J Natl Cancer Inst.1979. 62:1009～1012 15 Newmark HI，Wargovich S. Bruce WR. Colon cancer and dietary fat，phosphates and calcium; A hypothesis.J Natl Cancer Inst. 1984.72:1323～1325 16 Xue I_X, Newmark HI，Leung D. Epithelial cell hyperproliferation induced in the exocrine pancreas of mice by a Western-style diet. J Natl Cancer Inst. 1996.88;1586～1590 17 Xue I_X, I_ipkin M. Newmark Hl.Induced hyperpoliferanon in epithelial cells of mouse prostate by a Westernstyle diet. Carcinogenesis，1997. 18:995～999 18 Colston KW. Berger U, Combes RC. Possible role for vitamin D in controlling breast cancer cell proliferation.Lancet, 1989,1:188～191 19 Koga M，Eisman JA. Sutherland RI_. Regulation of epidermal growth factor receptor levels by 1.25-dihydroxyvitamin Da in human bresat cancer cells. Cancer Res. 1988.48:2734～2739
计量
- 文章访问数: 1058
- HTML全文浏览量: 27
- PDF下载量: 284
下载:
