血液肿瘤细胞凋亡形态学观察的改良荧光染色法

A Modified Method of Fluorescent Staining for the Apoptosis Detection fron Blood Tumor Cells

摘要: 改良了观察血液肿瘤细胞凋亡形态学的荧光染色法。其程序包括细胞固定、制片、染色、现察等步骤，简单易行；整个细胞凋亡检测过程在2h内即可完成，且检出率极高，甚至在细胞凋亡发生率仅为1%的情况下，仍可快速检出。因此，该染色法具有方便、快速、实用性强的特点。适用于常规实验室细胞凋亡形态学观察。

Abstract: A very useful method,fluorescent（Hoechst33258）staining is improved to detect apoptosis in suspension cultured HL－60,,K562 cells in vitro.The procedure involving cell fixating,making slide,staining and detecting can be finished in 2h,and this allows a survey of several thousand cells by a quick microscopic scan even when the apoptosis rate is extremely low（ l%）.Consequently,this method should be an easy,fast,and highly reliable system for routine laboratory detecting of apoptosis in suspension cultured cancer cells.