Abstract: bladder cancer patients were evaluated for β₂-microglobulin expression by means of PAP method and 28 of them for also ER and PR. Result showed that ERwas positive in 10 cases(35.7%),PR positive in 8 cases (28. 6%)and β₂-microglobulin positive in 15 cases (42.9%).There was a positive correlation between cancer histologic grading, clinical staging and ER, PR content, while a negative correlation existed between the former and β₂-microglobulin. In 5 year follow-up study, the recurrence and mortality rate of ER(+ ),and PR (+ ) patient were higher than that of ER(-), PR(-) patient, while that of β₂-microglobulin(+) patient were lower than in β₂-microglobulin(-) patient. This suggests that ER and PR are present in bladder cancer tissue, they might play a role in evaluating the diagnosis and prognosis of bladder tumor.In addition,ER and PR two groups of marker opposite to each other. on the basisof and β₂-microglobulin are two groups of marker opposite to each other. On the basis of this study, it stands to reason that the adjuvant hormonal therapy is tried in ER, PR positive bladder cancer patient.