前列腺素E<sub>1</sub>和亚硒酸钠对A<sub>549</sub>肺癌细胞DNA含量的影响

前列腺素E₁和亚硒酸钠对A₅₄₉肺癌细胞DNA含量的影响

摘要: 本实验观察了PGE₁和Na₂SeO₃对体外培养的A₅₄₉细胞DNA含量的影响。实验分为四组：Ⅰ组作为对照组，Ⅱ，Ⅲ，Ⅳ组培养基内分别加入10ngPGE₁/ml，10ngPGE₁和1μgSe/ml，1μgSe/ml。使用显微分光光度计测量结果表明，Ⅱ组（10ngPGE₁/ml）和Ⅳ组（1μgSe/ml）A₅₄₉细胞内DNA含量明显降低。通过测定单个细胞的DNA含量，我们发现PGE：和Se作用后A₅₄₉细胞各周期比率发生了改变。Ⅳ组的组方图峰值左移。我们对PFE₁和Na₂SeO₃可能的抑制机制进行了讨论。

Abstract: The effect of prostaglandin E₁ and Sodium Selenite on the DNA content in A₅₄₉ cells was investigated in vitro. The A_(549) cells were divided into four groups: Groupl was Cultured in the medium alone for control. Group Ⅱ, Ⅲ, Ⅳ were culturet in the medium addedwith 10ngPGE_1/ml, 10ngPGE_1 10ngPGE and 1μgSe/ml, 1μgSe/ml, respectively. By means of UNIVAR microscope photometer, it was found that DNA content in A_(549) cells treated with 10ngPGE_1/ml (group Ⅱ) or lμgSe/ml (groupⅣ) concentration was decreased(P0.01). By the determination of DNA content in each individual cell, we found that the location of cells in each phase compartment was changed. The peak in group Ⅳ moved to the left. The possible mechanisms of the inhibitory effect ty PGE_1 and Na_2SeO_ were discussed.