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前列腺素E1和亚硒酸钠对A549肺癌细胞DNA含量的影响

刘志芳, 田鸿生

刘志芳, 田鸿生. 前列腺素E1和亚硒酸钠对A549肺癌细胞DNA含量的影响[J]. 肿瘤防治研究, 1991, 18(1): 36-38.
引用本文: 刘志芳, 田鸿生. 前列腺素E1和亚硒酸钠对A549肺癌细胞DNA含量的影响[J]. 肿瘤防治研究, 1991, 18(1): 36-38.
Liu Zhifang, . THE EFFECT OF PROSTAGLANDIN E1 AND SODIUM SELENITE ON THE DNA CONTENT IN A549 CELLS IN VITRO[J]. Cancer Research on Prevention and Treatment, 1991, 18(1): 36-38.
Citation: Liu Zhifang, . THE EFFECT OF PROSTAGLANDIN E1 AND SODIUM SELENITE ON THE DNA CONTENT IN A549 CELLS IN VITRO[J]. Cancer Research on Prevention and Treatment, 1991, 18(1): 36-38.

前列腺素E1和亚硒酸钠对A549肺癌细胞DNA含量的影响

THE EFFECT OF PROSTAGLANDIN E1 AND SODIUM SELENITE ON THE DNA CONTENT IN A549 CELLS IN VITRO

  • 摘要: 本实验观察了PGE1和Na2SeO3对体外培养的A549细胞DNA含量的影响。实验分为四组:Ⅰ组作为对照组,Ⅱ,Ⅲ,Ⅳ组培养基内分别加入10ngPGE1/ml,10ngPGE1和1μgSe/ml,1μgSe/ml。使用显微分光光度计测量结果表明,Ⅱ组(10ngPGE1/ml)和Ⅳ组(1μgSe/ml)A549细胞内DNA含量明显降低。通过测定单个细胞的DNA含量,我们发现PGE:和Se作用后A549细胞各周期比率发生了改变。Ⅳ组的组方图峰值左移。我们对PFE1和Na2SeO3可能的抑制机制进行了讨论。

     

    Abstract: The effect of prostaglandin E1 and Sodium Selenite on the DNA content in A549 cells was investigated in vitro. The A_(549) cells were divided into four groups: Groupl was Cultured in the medium alone for control. Group Ⅱ, Ⅲ, Ⅳ were culturet in the medium addedwith 10ngPGE_1/ml, 10ngPGE_1 10ngPGE and 1μgSe/ml, 1μgSe/ml, respectively. By means of UNIVAR microscope photometer, it was found that DNA content in A_(549) cells treated with 10ngPGE_1/ml (group Ⅱ) or lμgSe/ml (groupⅣ) concentration was decreased(P0.01). By the determination of DNA content in each individual cell, we found that the location of cells in each phase compartment was changed. The peak in group Ⅳ moved to the left. The possible mechanisms of the inhibitory effect ty PGE_1 and Na_2SeO_ were discussed.

     

  • 1. Thomas DR et al, Characteristics of HT-29. T. Surg, Res.1974;16:463-465
    2. Schnauzer GN,(Abstracts in Munch, med. wschr, 1985; 30:731
    3. 田鸿生等:肿瘤防治研究1985;12:125
    4. Pattison R. Press. 1979, London cademic
    5. 王登顺:国外医学(肿瘤学分册)1987;3: 136-141
    6. 黄威权。国外医学(肿瘤学分册)1985; 4:208-212
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出版历程
  • 刊出日期:  1991-03-04

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