甲基硝基亚硝基胍（MNNG）转化的金仓鼠乳鼠肺细胞系（BHLB4）的细胞周期与同步化研究
CELL CYCLE ANALYSIS AND PARTIAL SYNCHRONIZATION OF AN-METHYL-N'-N-ITOR N NITROSOGUANIDINE(MNNG) TRANSFORMEDBABY SYRIAN HAMSTER LUNG CELL LINE(BHLB4)
-
摘要: 金仓鼠细胞培养系统已证明是研究癌变原理的良好实验模型。我们曾报导用MNNG转化金仓鼠乳鼠肺细胞,获得了一个恶性转化的细胞系（BHLB4）,并进行了一系列生物学特性研究和染色体核型分析。Abstract: The cell cycle analysis and partialsynchron}zation of a MNNG-transformed cellline(BHLB4)were carried out and the results obtained were as follows:cell generation time (GT) 11,6 hr, s 6.2 hr G2 1.3 hr, M1.8 hr G 2.3 hr, When this cellline was synchronized by 2x107 MMTX treatment for 14hr, the labelleu index reached:a peak of about 6o-70%1 hr, after release and the mitot;cintlex reached a peak of 3 -10%7 hr, after release, and could be further increased to about 20% in the prosence of 0.2ug/ml vincristme for 2, hours beefore harvesting.
-
-
[1] .李申德等实验生物学报12(3):195 -202 1979 [2] .李申德等实验生物学报13(1):29-41 1980 [3] .Shende Li ef: al.Cytogenet, Cell Genet 33 295一302 1982 [4] Shende Li et al.Anticancer Res, 3:117--120 1833 [5] .Thomes D. B. The kinetics of cell product- ion in."The cells in Medical Sciences 2: 201一243 1974 [6] .Cleaver J.E.Thymidine metabolism and cel Kinetics North-Hlollaap Publishing ComP13 any Amsterdam 108一118 1.37 [7] .旷健等中华肿瘤杂志5(3):101一163 198- [8] .Yunis J.J.Progress in clinical patiolony 7: 287一283 1978 [9] .Camargo M.et al.Human Genetics 54:47一 58 1980 [10] .Bostoch G.J.et al,Exp,Cell Res.08:103一 168 1977
计量
- 文章访问数: 882
- HTML全文浏览量: 21
- PDF下载量: 172
下载:
