交链孢酚单甲醚对人淋巴细胞增殖反应的影响

EFFECT OF AME ON PROLIFERATIVE RESPONSE IN HUMAN LYMPHOCYTES

摘要: 本实验运用PHA诱导的淋巴细胞增殖³H—TdR掺入法,对食管癌高发区河南省林县粮食中分离的优势污染菌互隔交链孢霉(亦称链格孢菌)的毒素之一——交链孢酚单甲醚(AME)的免疫抑制作用进行了研究。实验结果表明:AME能明显抑制淋巴细胞对³H—TdR的摄取,在浓度为25、50、75和100μg/ml,其抑制率分别是76.9%、89.2%、91.3%和94.9%,与对照组相比有显著性意义(P＜0.01),且存在一定的剂量—效应关系。结果提示AME可能抑制细胞免疫功能。

Abstract: In this paper, we investigated the suppressive effect of AME (alternariol monomethyl ether) on proliferative response in human lymphocytes by use of PHA-induced lymphocyte proliferation SH-TdR incorporation method. The results showed: AME had a significant inhibitory effect on SH-TdR incorporation into lymphocytes. As the concentration of AME was 25ug/ml, 50ug/ml, 75ug/ml and 100μg/ml, its suppressive ratio was 76.9%, 89.2%, 91.3% and 94.9% respectively. The effect of AME was significantly different from control (P0.01), and had does-dependent fashion (Y=12.13-0.10X,r=-0.927, P0.05, alone). This result suggests that AME could inhibit cellular immune and affect the AME-induced carcinogenesis.