宫颈癌组织中HPV<sub>16</sub>DNA基因同源序列的检测

宫颈癌组织中HPV₁₆DNA基因同源序列的检测

摘要: 206例不同病理诊断的宫颈脱落细胞和宫颈组织DNA,同探针杂交,检测HPV₁₆DNA同源性序列。结果表明:HPV₁₆DNA探针同宫颈癌脱落细胞杂交（65.2%）和同宫颈癌组织DNA杂交（66.7%）,其杂交率无显著差别（P＜0.05）。宫颈炎脱落细胞DNA和宫颈炎组织DNA与HPV₁₆DNA探针杂交率间的差异也无统计学意义。然而,宫颈癌和宫颈炎之间无论是脱落细胞或是组织DNA同探针杂交有显著差异（前者为65.2—66.7%,后者为26.1—28.5%）P＜0.01。宫颈癌和宫颈炎DNA经酶切电泳后,Southern blot杂交结果提示HPV₁₆DNA整合在癌组织基因组中。

Abstract: DNA extracted from the cervical tissues and cervical decidual, cell were detected by hybridization of nucleic acid with the probe of (α—32p)—dCTP labeled 7.9Kb HPV DNA. The positive rate of HPV16 DNA of decidual cell DNA was 65.2%, of cervical cancer tissues DNA was 66.7% and had no significant difference, but differences between cervical cancer and cervicitis, eithcr decidual cell DNA or tissue DNA hybridized with probe all had significant difference (former 65.2—66.7%, latter 26.1—28.5%), P0.01. The Physical state of HPV16 DNA in cervical cancer southern blot and twe-dimentional electrophoresis were evaluated and showed that HPV16 DNAwas integrated in cervical cancer cell genome.